Richard G. Fehon

Research Summary
The Fehon laboratory studies the organization and function of signaling pathway components at the cell cortex, particularly relating to the Hippo growth control pathway. In addition to their known role in organizing receptors and downstream effectors into functional signaling complexes, such organized complexes function to integrate signaling activities from multiple pathways and to segregate simultaneous but distinct functions of a single pathway. We study this question in Drosophila because of the utility of this system for studying the functions of individual genes, and for examining the functional interactions between different genes that work together in a particular cellular or developmental process.
Cell signaling, Development, Hippo pathway, Epithelial polarity, Microscopy, Genetics
  • Yale University, New Haven, CT, Postdoctoral Developmental Genetics 1991
  • University of Washington, Seattle, WA, PhD Developmental Biology 1986
  • Duke University, Durham, NC, BS Zoology 1980
  1. Tokamov SA, Su T, Ullyot A, Fehon RG. Negative feedback couples Hippo pathway activation with Kibra degradation independent of Yorkie-mediated transcription. Elife. 2021 02 08; 10. View in: PubMed

  2. Rich A, Fehon RG, Glotzer M. Rho1 activation recapitulates early gastrulation events in the ventral, but not dorsal, epithelium of Drosophila embryos. Elife. 2020 11 17; 9. View in: PubMed

  3. Yee WB, Delaney PM, Vanderzalm PJ, Ramachandran S, Fehon RG. The CAF-1 complex couples Hippo pathway target gene expression and DNA replication. Mol Biol Cell. 2019 11 01; 30(23):2929-2942. View in: PubMed

  4. Xu J, Su T, Tokamov SA, Fehon RG. Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs. Methods Mol Biol. 2019; 1893:53-59. View in: PubMed

  5. Xu J, Vanderzalm PJ, Ludwig M, Su T, Tokamov SA, Fehon RG. Yorkie Functions at the Cell Cortex to Promote Myosin Activation in a Non-transcriptional Manner. Dev Cell. 2018 08 06; 46(3):271-284.e5. View in: PubMed

  6. Matakatsu H, Blair SS, Fehon RG. Size does matter! Cell Cycle. 2017 05 19; 16(10):907-908. View in: PubMed

  7. Su T, Ludwig MZ, Xu J, Fehon RG. Kibra and Merlin Activate the Hippo Pathway Spatially Distinct from and Independent of Expanded. Dev Cell. 2017 03 13; 40(5):478-490.e3. View in: PubMed

  8. Matakatsu H, Blair SS, Fehon RG. The palmitoyltransferase Approximated promotes growth via the Hippo pathway by palmitoylation of Fat. J Cell Biol. 2017 Jan 02; 216(1):265-277. View in: PubMed

  9. Zhang Y, Wang X, Matakatsu H, Fehon R, Blair SS. The novel SH3 domain protein Dlish/CG10933 mediates fat signaling in Drosophila by binding and regulating Dachs. Elife. 2016 10 03; 5. View in: PubMed

  10. Sherrard KM, Fehon RG. The transmembrane protein Crumbs displays complex dynamics during follicular morphogenesis and is regulated competitively by Moesin and aPKC. Development. 2015 May 15; 142(10):1869-78. View in: PubMed

  11. Gavilan HS, Kulikauskas RM, Gutmann DH, Fehon RG. In vivo functional analysis of the human NF2 tumor suppressor gene in Drosophila. PLoS One. 2014; 9(3):e90853. View in: PubMed

  12. Hall S, Bone C, Oshima K, Zhang L, McGraw M, Lucas B, Fehon RG, Ward RE. Macroglobulin complement-related encodes a protein required for septate junction organization and paracellular barrier function in Drosophila. Development. 2014 Feb; 141(4):889-98. View in: PubMed

  13. Neisch AL, Formstecher E, Fehon RG. Conundrum, an ARHGAP18 orthologue, regulates RhoA and proliferation through interactions with Moesin. Mol Biol Cell. 2013 May; 24(9):1420-33. View in: PubMed

  14. Boggiano JC, Fehon RG. Growth control by committee: intercellular junctions, cell polarity, and the cytoskeleton regulate Hippo signaling. Dev Cell. 2012 Apr 17; 22(4):695-702. View in: PubMed

  15. Boggiano JC, Vanderzalm PJ, Fehon RG. Tao-1 phosphorylates Hippo/MST kinases to regulate the Hippo-Salvador-Warts tumor suppressor pathway. Dev Cell. 2011 Nov 15; 21(5):888-95. View in: PubMed

  16. Oshima K, Fehon RG. Analysis of protein dynamics within the septate junction reveals a highly stable core protein complex that does not include the basolateral polarity protein Discs large. J Cell Sci. 2011 Aug 15; 124(Pt 16):2861-71. View in: PubMed

  17. Neisch AL, Fehon RG. Ezrin, Radixin and Moesin: key regulators of membrane-cortex interactions and signaling. Curr Opin Cell Biol. 2011 Aug; 23(4):377-82. View in: PubMed

  18. Nilton A, Oshima K, Zare F, Byri S, Nannmark U, Nyberg KG, Fehon RG, Uv AE. Crooked, coiled and crimpled are three Ly6-like proteins required for proper localization of septate junction components. Development. 2010 Jul; 137(14):2427-37. View in: PubMed

  19. Neisch AL, Speck O, Stronach B, Fehon RG. Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. J Cell Biol. 2010 Apr 19; 189(2):311-23. View in: PubMed

  20. Bense?or LB, Barlan K, Rice SE, Fehon RG, Gelfand VI. Microtubule-mediated transport of the tumor-suppressor protein Merlin and its mutants. Proc Natl Acad Sci U S A. 2010 Apr 20; 107(16):7311-6. View in: PubMed

  21. Fehon RG, McClatchey AI, Bretscher A. Organizing the cell cortex: the role of ERM proteins. Nat Rev Mol Cell Biol. 2010 Apr; 11(4):276-87. View in: PubMed

  22. Hughes SC, Formstecher E, Fehon RG. Sip1, the Drosophila orthologue of EBP50/NHERF1, functions with the sterile 20 family kinase Slik to regulate Moesin activity. J Cell Sci. 2010 Apr 01; 123(Pt 7):1099-107. View in: PubMed

  23. McClatchey AI, Fehon RG. Merlin and the ERM proteins--regulators of receptor distribution and signaling at the cell cortex. Trends Cell Biol. 2009 May; 19(5):198-206. View in: PubMed

  24. Neisch A, Fehon RG. FERMing up the plasma membrane. Dev Cell. 2008 Feb; 14(2):154-6. View in: PubMed

  25. Hughes SC, Fehon RG. Understanding ERM proteins--the awesome power of genetics finally brought to bear. Curr Opin Cell Biol. 2007 Feb; 19(1):51-6. View in: PubMed

  26. Li Q, Nance MR, Kulikauskas R, Nyberg K, Fehon R, Karplus PA, Bretscher A, Tesmer JJ. Self-masking in an intact ERM-merlin protein: an active role for the central alpha-helical domain. J Mol Biol. 2007 Feb 02; 365(5):1446-59. View in: PubMed

  27. Hughes SC, Fehon RG. Phosphorylation and activity of the tumor suppressor Merlin and the ERM protein Moesin are coordinately regulated by the Slik kinase. J Cell Biol. 2006 Oct 23; 175(2):305-13. View in: PubMed

  28. Fehon R. Cell biology: polarity bites. Nature. 2006 Aug 03; 442(7102):519-20. View in: PubMed

  29. Maitra S, Kulikauskas RM, Gavilan H, Fehon RG. The tumor suppressors Merlin and Expanded function cooperatively to modulate receptor endocytosis and signaling. Curr Biol. 2006 Apr 04; 16(7):702-9. View in: PubMed

  30. Formstecher E, Aresta S, Collura V, Hamburger A, Meil A, Trehin A, Reverdy C, Betin V, Maire S, Brun C, Jacq B, Arpin M, Bellaiche Y, Bellusci S, Benaroch P, Bornens M, Chanet R, Chavrier P, Delattre O, Doye V, Fehon R, Faye G, Galli T, Girault JA, Goud B, de Gunzburg J, Johannes L, Junier MP, Mirouse V, Mukherjee A, Papadopoulo D, Perez F, Plessis A, Ross? C, Saule S, Stoppa-Lyonnet D, Vincent A, White M, Legrain P, Wojcik J, Camonis J, Daviet L. Protein interaction mapping: a Drosophila case study. Genome Res. 2005 Mar; 15(3):376-84. View in: PubMed

  31. Genova JL, Fehon RG. Neuroglian, Gliotactin, and the Na+/K+ ATPase are essential for septate junction function in Drosophila. J Cell Biol. 2003 Jun 09; 161(5):979-89. View in: PubMed

  32. Speck O, Hughes SC, Noren NK, Kulikauskas RM, Fehon RG. Moesin functions antagonistically to the Rho pathway to maintain epithelial integrity. Nature. 2003 Jan 02; 421(6918):83-7. View in: PubMed

  33. Bretscher A, Edwards K, Fehon RG. ERM proteins and merlin: integrators at the cell cortex. Nat Rev Mol Cell Biol. 2002 Aug; 3(8):586-99. View in: PubMed

  34. Tepass U, Tanentzapf G, Ward R, Fehon R. Epithelial cell polarity and cell junctions in Drosophila. Annu Rev Genet. 2001; 35:747-84. View in: PubMed

  35. Ward RE, Schweizer L, Lamb RS, Fehon RG. The protein 4.1, ezrin, radixin, moesin (FERM) domain of Drosophila Coracle, a cytoplasmic component of the septate junction, provides functions essential for embryonic development and imaginal cell proliferation. Genetics. 2001 Sep; 159(1):219-28. View in: PubMed

  36. LaJeunesse DR, McCartney BM, Fehon RG. A systematic screen for dominant second-site modifiers of Merlin/NF2 phenotypes reveals an interaction with blistered/DSRF and scribbler. Genetics. 2001 Jun; 158(2):667-79. View in: PubMed

  37. Lim DJ, Rubenstein AE, Evans DG, Jacks T, Seizinger BG, Baser ME, Beebe D, Brackmann DE, Chiocca EA, Fehon RG, Giovannini M, Glazer R, Gusella JF, Gutmann DH, Korf B, Lieberman F, Martuza R, McClatchey AI, Parry DM, Pulst SM, Ramesh V, Ramsey WJ, Ratner N, Rutkowski JL, Ruttledge M, Weinstein DE. Advances in neurofibromatosis 2 (NF2): a workshop report. J Neurogenet. 2000 Jun; 14(2):63-106. View in: PubMed

  38. Genova JL, Jong S, Camp JT, Fehon RG. Functional analysis of Cdc42 in actin filament assembly, epithelial morphogenesis, and cell signaling during Drosophila development. Dev Biol. 2000 May 01; 221(1):181-94. View in: PubMed

  39. McCartney BM, Kulikauskas RM, LaJeunesse DR, Fehon RG. The neurofibromatosis-2 homologue, Merlin, and the tumor suppressor expanded function together in Drosophila to regulate cell proliferation and differentiation. Development. 2000 Mar; 127(6):1315-24. View in: PubMed

  40. Lamb RS, Ward RE, Schweizer L, Fehon RG. Drosophila coracle, a member of the protein 4.1 superfamily, has essential structural functions in the septate junctions and developmental functions in embryonic and adult epithelial cells. Mol Biol Cell. 1998 Dec; 9(12):3505-19. View in: PubMed

  41. Chishti AH, Kim AC, Marfatia SM, Lutchman M, Hanspal M, Jindal H, Liu SC, Low PS, Rouleau GA, Mohandas N, Chasis JA, Conboy JG, Gascard P, Takakuwa Y, Huang SC, Benz EJ, Bretscher A, Fehon RG, Gusella JF, Ramesh V, Solomon F, Marchesi VT, Tsukita S, Tsukita S, Hoover KB, et al. The FERM domain: a unique module involved in the linkage of cytoplasmic proteins to the membrane. Trends Biochem Sci. 1998 Aug; 23(8):281-2. View in: PubMed

  42. LaJeunesse DR, McCartney BM, Fehon RG. Structural analysis of Drosophila merlin reveals functional domains important for growth control and subcellular localization. J Cell Biol. 1998 Jun 29; 141(7):1589-99. View in: PubMed

  43. Ward RE, Lamb RS, Fehon RG. A conserved functional domain of Drosophila coracle is required for localization at the septate junction and has membrane-organizing activity. J Cell Biol. 1998 Mar 23; 140(6):1463-73. View in: PubMed

  44. Fehon RG, LaJeunesse D, Lamb R, McCartney BM, Schweizer L, Ward RE. Functional studies of the protein 4.1 family of junctional proteins in Drosophila. Soc Gen Physiol Ser. 1997; 52:149-59. View in: PubMed

  45. Fehon RG, Oren T, LaJeunesse DR, Melby TE, McCartney BM. Isolation of mutations in the Drosophila homologues of the human Neurofibromatosis 2 and yeast CDC42 genes using a simple and efficient reverse-genetic method. Genetics. 1997 May; 146(1):245-52. View in: PubMed

  46. McCartney BM, Fehon RG. Distinct cellular and subcellular patterns of expression imply distinct functions for the Drosophila homologues of moesin and the neurofibromatosis 2 tumor suppressor, merlin. J Cell Biol. 1996 May; 133(4):843-52. View in: PubMed

  47. Rebay I, Fehon RG, Artavanis-Tsakonas S. Specific truncations of Drosophila Notch define dominant activated and dominant negative forms of the receptor. Cell. 1993 Jul 30; 74(2):319-29. View in: PubMed

  48. Kooh PJ, Fehon RG, Muskavitch MA. Implications of dynamic patterns of Delta and Notch expression for cellular interactions during Drosophila development. Development. 1993 Feb; 117(2):493-507. View in: PubMed

  49. Fehon RG, Dawson IA, Artavanis-Tsakonas S. A Drosophila homologue of membrane-skeleton protein 4.1 is associated with septate junctions and is encoded by the coracle gene. Development. 1994 Mar; 120(3):545-57. View in: PubMed

  50. Fehon RG, Schubiger G. Dissociation and sorting out of Drosophila imaginal disc cells. Dev Biol. 1985 Apr; 108(2):465-73. View in: PubMed

  51. Gauger A, Fehon RG, Schubiger G. Preferential binding of imaginal disk cells to embryonic segments of Drosophila. Nature. 1985 Jan 31-Feb 6; 313(6001):395-7. View in: PubMed

  52. Johansen KM, Fehon RG, Artavanis-Tsakonas S. The notch gene product is a glycoprotein expressed on the cell surface of both epidermal and neuronal precursor cells during Drosophila development. J Cell Biol. 1989 Nov; 109(5):2427-40. View in: PubMed

  53. Artavanis-Tsakonas S, Delidakis C, Fehon R, Hartley D, Herndon V, Johansen K, Markopoulou K, Preiss A, Rebay I, Scottgale N, et al. Notch and the molecular genetics of neuroblast segregation in Drosophila. Mol Reprod Dev. 1990 Sep; 27(1):23-7. View in: PubMed

  54. Fehon RG, Kooh PJ, Rebay I, Regan CL, Xu T, Muskavitch MA, Artavanis-Tsakonas S. Molecular interactions between the protein products of the neurogenic loci Notch and Delta, two EGF-homologous genes in Drosophila. Cell. 1990 May 04; 61(3):523-34. View in: PubMed

  55. Fehon RG, Johansen K, Rebay I, Artavanis-Tsakonas S. Complex cellular and subcellular regulation of notch expression during embryonic and imaginal development of Drosophila: implications for notch function. J Cell Biol. 1991 May; 113(3):657-69. View in: PubMed

  56. Artavanis-Tsakonas S, Delidakis C, Fehon RG. The Notch locus and the cell biology of neuroblast segregation. Annu Rev Cell Biol. 1991; 7:427-52. View in: PubMed

  57. Rebay I, Fleming RJ, Fehon RG, Cherbas L, Cherbas P, Artavanis-Tsakonas S. Specific EGF repeats of Notch mediate interactions with Delta and Serrate: implications for Notch as a multifunctional receptor. Cell. 1991 Nov 15; 67(4):687-99. View in: PubMed

  58. Xu T, Caron LA, Fehon RG, Artavanis-Tsakonas S. The involvement of the Notch locus in Drosophila oogenesis. Development. 1992 Aug; 115(4):913-22. View in: PubMed