Sampriti Mukherjee

Assistant Professor
Research Summary
Our overarching goal is to understand how bacteria decode and integrate self-generated and environmentally-derived stimuli to control transitions between individual and collective behaviors. Bacterial responses to self-generated and exogenous stimuli influence their survival, persistence in particular niches, and lifestyle transitions, such as alterations between being free-swimming (individual behavior) or existing in structured antibiotic-resistant communities called biofilms (collective behavior). How the information encoded in multiple sensory inputs is extracted and integrated to drive transitions between individual and collective behaviors is largely mysterious. We use bacterial genetics, molecular biology, biochemistry, microfluidics, fluorescence microscopy, and genome-scale studies to study fundamental questions about bacterial signal detection, signal relay, signal integration, and the consequences to collective behaviors. Ultimately, understanding how information encoded in diverse sensory inputs drives collective behaviors will be foundational for designing successful synthetic strategies to enhance or to inhibit biofilms and for developing novel therapeutic interventions.
Bacterial genetics, Bacterial sensory signal transduction, Biofilms, Molecular biology, Microscopy, Genomics
  • Princeton University, Princeton, NJ , USA, Postdoctoral Fellow Molecular Biology 08/2020
  • Indiana University, Bloomington, IN, USA, PhD Microbiology 10/2014
  • University of Calcutta, Kolkata, India, BS Microbiology 07/2007
  • University of Calcutta, Kolkata, India, MS Microbiology 06/2009
Awards & Honors
  • 2016 - 2019 Life Sciences Research Foundation Postdoctoral Fellowship
  • 2018 - 2023 NIH Pathway to Independence Award (K99/R00)
  1. Jiang Z, Nero T, Mukherjee S, Olson R, Yan J. Searching for the Secret of Stickiness: How Biofilms Adhere to Surfaces. Front Microbiol. 2021; 12:686793. View in: PubMed

  2. van Kessel JC, Mukherjee S. Another battle won in the phage-host arms race: Pseudomonas phage blocks quorum sensing regulator LasR. Mol Cell. 2021 02 04; 81(3):420-422. View in: PubMed

  3. Mukherjee S, Jemielita M, Stergioula V, Tikhonov M, Bassler BL. Photosensing and quorum sensing are integrated to control Pseudomonas aeruginosa collective behaviors. PLoS Biol. 2019 12; 17(12):e3000579. View in: PubMed

  4. Mukherjee S, Bassler BL. Bacterial quorum sensing in complex and dynamically changing environments. Nat Rev Microbiol. 2019 06; 17(6):371-382. View in: PubMed

  5. Yan J, Moreau A, Khodaparast S, Perazzo A, Feng J, Fei C, Mao S, Mukherjee S, Košmrlj A, Wingreen NS, Bassler BL, Stone HA. Bacterial Biofilm Material Properties Enable Removal and Transfer by Capillary Peeling. Adv Mater. 2019 01; 31(4):e1807586. View in: PubMed

  6. Yan J, Moreau A, Khodaparast S, Perazzo A, Feng J, Fei C, Mao S, Mukherjee S, Košmrlj A, Wingreen NS, Bassler BL, Stone HA. Bacterial Biofilm Material Properties Enable Removal and Transfer by Capillary Peeling. Adv Mater. 2018 Nov; 30(46):e1804153. View in: PubMed

  7. Mukherjee S, Moustafa DA, Stergioula V, Smith CD, Goldberg JB, Bassler BL. The PqsE and RhlR proteins are an autoinducer synthase-receptor pair that control virulence and biofilm development in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2018 10 02; 115(40):E9411-E9418. View in: PubMed

  8. Altegoer F, Mukherjee S, Steinchen W, Bedrunka P, Linne U, Kearns DB, Bange G. FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis. Sci Rep. 2018 08 01; 8(1):11552. View in: PubMed

  9. Mukherjee S, Moustafa D, Smith CD, Goldberg JB, Bassler BL. The RhlR quorum-sensing receptor controls Pseudomonas aeruginosa pathogenesis and biofilm development independently of its canonical homoserine lactone autoinducer. PLoS Pathog. 2017 Jul; 13(7):e1006504. View in: PubMed

  10. Paczkowski JE, Mukherjee S, McCready AR, Cong JP, Aquino CJ, Kim H, Henke BR, Smith CD, Bassler BL. Flavonoids Suppress Pseudomonas aeruginosa Virulence through Allosteric Inhibition of Quorum-sensing Receptors. J Biol Chem. 2017 03 10; 292(10):4064-4076. View in: PubMed

  11. Høyland-Kroghsbo NM, Paczkowski J, Mukherjee S, Broniewski J, Westra E, Bondy-Denomy J, Bassler BL. Quorum sensing controls the Pseudomonas aeruginosa CRISPR-Cas adaptive immune system. Proc Natl Acad Sci U S A. 2017 01 03; 114(1):131-135. View in: PubMed

  12. Mukherjee S, Oshiro RT, Yakhnin H, Babitzke P, Kearns DB. FliW antagonizes CsrA RNA binding by a noncompetitive allosteric mechanism. Proc Natl Acad Sci U S A. 2016 08 30; 113(35):9870-5. View in: PubMed

  13. Abbott ZD, Flynn KJ, Byrne BG, Mukherjee S, Kearns DB, Swanson MS. csrT Represents a New Class of csrA-Like Regulatory Genes Associated with Integrative Conjugative Elements of Legionella pneumophila. J Bacteriol. 2016 02 01; 198(3):553-64. View in: PubMed

  14. Mukherjee S, Bree AC, Liu J, Patrick JE, Chien P, Kearns DB. Adaptor-mediated Lon proteolysis restricts Bacillus subtilis hyperflagellation. Proc Natl Acad Sci U S A. 2015 Jan 06; 112(1):250-5. View in: PubMed

  15. Mukherjee S, Kearns DB. The structure and regulation of flagella in Bacillus subtilis. Annu Rev Genet. 2014; 48:319-40. View in: PubMed

  16. Snyder AJ, Mukherjee S, Glass JK, Kearns DB, Mukhopadhyay S. The canonical twin-arginine translocase components are not required for secretion of folded green fluorescent protein from the ancestral strain of Bacillus subtilis. Appl Environ Microbiol. 2014 May; 80(10):3219-32. View in: PubMed

  17. Gao X, Mukherjee S, Matthews PM, Hammad LA, Kearns DB, Dann CE. Functional characterization of core components of the Bacillus subtilis cyclic-di-GMP signaling pathway. J Bacteriol. 2013 Nov; 195(21):4782-92. View in: PubMed

  18. Mukherjee S, Babitzke P, Kearns DB. FliW and FliS function independently to control cytoplasmic flagellin levels in Bacillus subtilis. J Bacteriol. 2013 Jan; 195(2):297-306. View in: PubMed

  19. Mukherjee S, Yakhnin H, Kysela D, Sokoloski J, Babitzke P, Kearns DB. CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis. Mol Microbiol. 2011 Oct; 82(2):447-61. View in: PubMed