Research Summary
The Kron laboratory is a diverse and collaborative group of cell biologists, geneticists, biochemists, chemists and computer scientists. Our current basic research and technology efforts include 1) defining roles for chromatin dynamics and cell cycle regulation in DNA damage checkpoint response and cellular senescence, 2) dissecting cross-talk between metabolism and DNA damage response, 3) developing novel molecular assays to interrogate cell signaling in cancer, and 4) implementing novel mass spectrometry approaches to enable quantitative proteomics. We also pursue translational projects directed at 1) discovering inhibitors of cellular response to DNA double strand breaks as an approach to radiosensitization, 2) examining DNA damage and repair in tissues and tumors, and 3) exploiting DNA damage responses to induce anti-tumor immune responses.
Senescence, Cell, Checkpoints, Cell Cycle, Immunotherapy, Radiotherapy, DNA Repair, Systems Biology
  • Whitehead Institute, Cambridge MA, Genetics 12/1995
  • Stanford University, Stanford CA, PhD Cell Biology 06/1990
  • Stanford University, Stanford CA, MD Medicine 06/1990
  • University of Pennsylvania, Philadelphia PA, MSE Bioengineering 05/1983
  • University of Pennsylvania, Philadelphia PA, BA Biochemistry 05/1982
  1. Lakiza O, Lutze J, Vogle A, Williams J, Abukdheir A, Miller P, Liao C', Pitroda SP, Martinez C, Olivas A, Setia N, Kron SJ, Weichselbaum RR, Keutgen XM. Loss of MEN1 function impairs DNA repair capability of pancreatic neuroendocrine tumors. Endocr Relat Cancer. 2022 03 21; 29(4):225-239. View in: PubMed

  2. Lutze J, Warrington SE, Kron SJ. TdT-dUTP DSB End Labeling (TUDEL), for Specific, Direct In Situ Labeling of DNA Double Strand Breaks. Methods Mol Biol. 2022; 2394:299-317. View in: PubMed

  3. Krishnan M, Senagolage MD, Baeten JT, Wolfgeher DJ, Khan S, Kron SJ, McNerney ME. Genomic studies controvert the existence of the CUX1 p75 isoform. Sci Rep. 2022 01 07; 12(1):151. View in: PubMed

  4. Samper-Martín B, Sarrias A, Lázaro B, Pérez-Montero M, Rodríguez-Rodríguez R, Ribeiro MPC, Bañón A, Wolfgeher D, Jessen HJ, Alsina B, Clotet J, Kron SJ, Saiardi A, Jiménez J, Bru S. Polyphosphate degradation by Nudt3-Zn2+ mediates oxidative stress response. Cell Rep. 2021 11 16; 37(7):110004. View in: PubMed

  5. Jelenska J, Lee J, Manning AJ, Wolfgeher DJ, Ahn Y, Walters-Marrah G, Lopez IE, Garcia L, McClerklin SA, Michelmore RW, Kron SJ, Greenberg JT. Pseudomonas syringae effector HopZ3 suppresses the bacterial AvrPto1-tomato PTO immune complex via acetylation. PLoS Pathog. 2021 11; 17(11):e1010017. View in: PubMed

  6. Imgruet MK, Lutze J, An N, Hu B, Khan S, Kurkewich J, Martinez TC, Wolfgeher D, Gurbuxani SK, Kron SJ, McNerney ME. Loss of a 7q gene, CUX1, disrupts epigenetically driven DNA repair and drives therapy-related myeloid neoplasms. Blood. 2021 09 02; 138(9):790-805. View in: PubMed

  7. Levin M, Flor AC, Snyder H, Kron SJ, Schwartz D. UltraPlex Hapten-Based Multiplexed Fluorescent Immunohistochemistry. Methods Mol Biol. 2021; 2350:267-287. View in: PubMed

  8. Phillips EH, Scholten D, Flor AC, Kron SJ, Lee SS. Multiplexed Tissue Tomography. Methods Mol Biol. 2021; 2350:77-93. View in: PubMed

  9. Ebenezer DL, Ramchandran R, Fu P, Mangio LA, Suryadevara V, Ha AW, Berdyshev E, Van Veldhoven PP, Kron SJ, Schumacher F, Kleuser B, Natarajan V. Nuclear Sphingosine-1-phosphate Lyase Generated ?2-hexadecenal is A Regulator of HDAC Activity and Chromatin Remodeling in Lung Epithelial Cells. Cell Biochem Biophys. 2021 Sep; 79(3):575-592. View in: PubMed

  10. Imgruet MK, Lutze J, An NN, Hu B, Khan S, Kurkewich J, Martinez TC, Wolfgeher DJ, Gurbuxani S, Kron SJ, McNerney ME. Loss of a 7q gene, CUX1, disrupts epigenetic-driven DNA repair and drives therapy-related myeloid neoplasms. Blood. 2021 May 26. View in: PubMed

  11. Prasanna PG, Citrin DE, Hildesheim J, Ahmed MM, Venkatachalam S, Riscuta G, Xi D, Zheng G, Deursen JV, Goronzy J, Kron SJ, Anscher MS, Sharpless NE, Campisi J, Brown SL, Niedernhofer LJ, O'Loghlen A, Georgakilas AG, Paris F, Gius D, Gewirtz DA, Schmitt CA, Abazeed ME, Kirkland JL, Richmond A, Romesser PB, Lowe SW, Gil J, Mendonca MS, Burma S, Zhou D, Coleman CN. Therapy-Induced Senescence: Opportunities to Improve Anticancer Therapy. J Natl Cancer Inst. 2021 10 01; 113(10):1285-1298. View in: PubMed

  12. Flor A, Wolfgeher D, Li J, Hanakahi LA, Kron SJ. Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons. Cell Chem Biol. 2021 06 17; 28(6):776-787.e8. View in: PubMed

  13. Feder ZA, Ali A, Singh A, Krakowiak J, Zheng X, Bindokas VP, Wolfgeher D, Kron SJ, Pincus D. Subcellular localization of the J-protein Sis1 regulates the heat shock response. J Cell Biol. 2021 01 04; 220(1). View in: PubMed

  14. Shamirian A, Appelbe O, Zhang Q, Ganesh B, Kron SJ, Snee PT. A toolkit for bioimaging using near-infrared AgInS2/ZnS quantum dots. J Mater Chem B. 2015 Nov 07; 3(41):8188-8196. View in: PubMed

  15. Brinkman JA, Liu Y, Kron SJ. Small-molecule drug repurposing to target DNA damage repair and response pathways. Semin Cancer Biol. 2021 01; 68:230-241. View in: PubMed

  16. Betori RC, Liu Y, Mishra RK, Cohen SB, Kron SJ, Scheidt KA. Targeted Covalent Inhibition of Telomerase. ACS Chem Biol. 2020 03 20; 15(3):706-717. View in: PubMed

  17. Weissman Z, Pinsky M, Wolfgeher DJ, Kron SJ, Truman AW, Kornitzer D. Genetic analysis of Hsp70 phosphorylation sites reveals a role in Candida albicans cell and colony morphogenesis. Biochim Biophys Acta Proteins Proteom. 2020 03; 1868(3):140135. View in: PubMed

  18. Moreno NS, Liu J, Haas KM, Parker LL, Chakraborty C, Kron SJ, Hodges K, Miller LD, Langefeld C, Robinson PJ, Lelièvre SA, Vidi PA. The nuclear structural protein NuMA is a negative regulator of 53BP1 in DNA double-strand break repair. Nucleic Acids Res. 2019 11 04; 47(19):10475. View in: PubMed

  19. Saloura V, Izumchenko E, Zuo Z, Bao R, Korzinkin M, Ozerov I, Zhavoronkov A, Sidransky D, Bedi A, Hoque MO, Koeppen H, Keck MK, Khattri A, London N, Kotlov N, Fatima A, Vougiouklakis T, Nakamura Y, Lingen M, Agrawal N, Savage PA, Kron S, Kline J, Kowanetz M, Seiwert TY. Immune profiles in primary squamous cell carcinoma of the head and neck. Oral Oncol. 2019 09; 96:77-88. View in: PubMed

  20. Ricco N, Flor A, Wolfgeher D, Efimova EV, Ramamurthy A, Appelbe OK, Brinkman J, Truman AW, Spiotto MT, Kron SJ. Mevalonate pathway activity as a determinant of radiation sensitivity in head and neck cancer. Mol Oncol. 2019 09; 13(9):1927-1943. View in: PubMed

  21. Liu Y, Efimova EV, Ramamurthy A, Kron SJ. Repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence. J Cell Sci. 2019 07 01; 132(13). View in: PubMed

  22. Hernández-Ortega S, Sánchez-Botet A, Quandt E, Masip N, Gasa L, Verde G, Jiménez J, Levin RS, Rutaganira FU, Burlingame AL, Wolfgeher D, Ribeiro MPC, Kron SJ, Shokat KM, Clotet J. Phosphoregulation of the oncogenic protein regulator of cytokinesis 1 (PRC1) by the atypical CDK16/CCNY complex. Exp Mol Med. 2019 04 16; 51(4):1-17. View in: PubMed

  23. Wu D, Pepowski B, Takahashi S, Kron SJ. A cmap-enabled gene expression signature-matching approach identifies small-molecule inducers of accelerated cell senescence. BMC Genomics. 2019 Apr 15; 20(1):290. View in: PubMed

  24. Ishihara J, Ishihara A, Sasaki K, Lee SS, Williford JM, Yasui M, Abe H, Potin L, Hosseinchi P, Fukunaga K, Raczy MM, Gray LT, Mansurov A, Katsumata K, Fukayama M, Kron SJ, Swartz MA, Hubbell JA. Targeted antibody and cytokine cancer immunotherapies through collagen affinity. Sci Transl Med. 2019 04 10; 11(487). View in: PubMed

  25. Khan MK, Nasti TH, Buchwald ZS, Weichselbaum RR, Kron SJ. Repurposing Drugs for Cancer Radiotherapy: Early Successes and Emerging Opportunities. Cancer J. 2019 Mar/Apr; 25(2):106-115. View in: PubMed

  26. Efimova EV, Appelbe OK, Ricco N, Lee SS, Liu Y, Wolfgeher DJ, Collins TN, Flor AC, Ramamurthy A, Warrington S, Bindokas VP, Kron SJ. O-GlcNAcylation Enhances Double-Strand Break Repair, Promotes Cancer Cell Proliferation, and Prevents Therapy-Induced Senescence in Irradiated Tumors. Mol Cancer Res. 2019 06; 17(6):1338-1350. View in: PubMed

  27. Salvador Moreno N, Liu J, Haas KM, Parker LL, Chakraborty C, Kron SJ, Hodges K, Miller LD, Langefeld C, Robinson PJ, Lelièvre SA, Vidi PA. The nuclear structural protein NuMA is a negative regulator of 53BP1 in DNA double-strand break repair. Nucleic Acids Res. 2019 04 08; 47(6):2703-2715. View in: PubMed

  28. Ranoa DRE, Widau RC, Mallon S, Parekh AD, Nicolae CM, Huang X, Bolt MJ, Arina A, Parry R, Kron SJ, Moldovan GL, Khodarev NN, Weichselbaum RR. STING Promotes Homeostasis via Regulation of Cell Proliferation and Chromosomal Stability. Cancer Res. 2019 04 01; 79(7):1465-1479. View in: PubMed

  29. Xu J, Lee SS, Seo H, Pang L, Jun Y, Zhang RY, Zhang ZY, Kim P, Lee W, Kron SJ, Yeo Y. Quinic Acid-Conjugated Nanoparticles Enhance Drug Delivery to Solid Tumors via Interactions with Endothelial Selectins. Small. 2018 12; 14(50):e1803601. View in: PubMed

  30. Lee SS, Bindokas VP, Lingen MW, Kron SJ. Nondestructive, multiplex three-dimensional mapping of immune infiltrates in core needle biopsy. Lab Invest. 2019 09; 99(9):1400-1413. View in: PubMed

  31. Lee SS, Bindokas VP, Kron SJ. Multiplex Three-Dimensional Mapping of Macromolecular Drug Distribution in the Tumor Microenvironment. Mol Cancer Ther. 2019 01; 18(1):213-226. View in: PubMed

  32. Cheng X, Jobin-Robitaille O, Billon P, Buisson R, Niu H, Lacoste N, Abshiru N, Côté V, Thibault P, Kron SJ, Sung P, Brandl CJ, Masson JY, Côté J. Phospho-dependent recruitment of the yeast NuA4 acetyltransferase complex by MRX at DNA breaks regulates RPA dynamics during resection. Proc Natl Acad Sci U S A. 2018 10 02; 115(40):10028-10033. View in: PubMed

  33. Sánchez-Botet A, Gasa L, Quandt E, Hernández-Ortega S, Jiménez J, Mezquita P, Carrasco-García MÀ, Kron SJ, Vidal A, Villanueva A, Ribeiro MPC, Clotet J. The atypical cyclin CNTD2 promotes colon cancer cell proliferation and migration. Sci Rep. 2018 08 07; 8(1):11797. View in: PubMed

  34. Fowler JL, Lee SS, Wesner ZC, Olehnik SK, Kron SJ, Hara M. Three-Dimensional Analysis of the Human Pancreas. Endocrinology. 2018 03 01; 159(3):1393-1400. View in: PubMed

  35. Appelbe OK, Kim BK, Rymut N, Wang J, Kron SJ, Yeo Y. Radiation-enhanced delivery of plasmid DNA to tumors utilizing a novel PEI polyplex. Cancer Gene Ther. 2018 08; 25(7-8):196-206. View in: PubMed

  36. Lee SS, Bindokas VP, Kron SJ. Multiplex three-dimensional optical mapping of tumor immune microenvironment. Sci Rep. 2017 12 05; 7(1):17031. View in: PubMed

  37. Flor AC, Wolfgeher D, Wu D, Kron SJ. A signature of enhanced lipid metabolism, lipid peroxidation and aldehyde stress in therapy-induced senescence. Cell Death Discov. 2017; 3:17075. View in: PubMed

  38. Manning AJ, Lee J, Wolfgeher DJ, Kron SJ, Greenberg JT. Simple strategies to enhance discovery of acetylation post-translational modifications by quadrupole-orbitrap LC-MS/MS. Biochim Biophys Acta Proteins Proteom. 2018 Feb; 1866(2):224-229. View in: PubMed

  39. Efimova EV, Ricco N, Labay E, Mauceri HJ, Flor AC, Ramamurthy A, Sutton HG, Weichselbaum RR, Kron SJ. HMG-CoA Reductase Inhibition Delays DNA Repair and Promotes Senescence After Tumor Irradiation. Mol Cancer Ther. 2018 02; 17(2):407-418. View in: PubMed

  40. Appelbe OK, Moynihan KD, Flor A, Rymut N, Irvine DJ, Kron SJ. Radiation-enhanced delivery of systemically administered amphiphilic-CpG oligodeoxynucleotide. J Control Release. 2017 Nov 28; 266:248-255. View in: PubMed

  41. Gasa L, Sanchez-Botet A, Quandt E, Hernández-Ortega S, Jiménez J, Carrasco-García MA, Simonetti S, Kron SJ, Ribeiro MP, Nadal E, Villanueva A, Clotet J. A systematic analysis of orphan cyclins reveals CNTD2 as a new oncogenic driver in lung cancer. Sci Rep. 2017 08 31; 7(1):10228. View in: PubMed

  42. Bar-Yosef H, Vivanco Gonzalez N, Ben-Aroya S, Kron SJ, Kornitzer D. Chemical inhibitors of Candida albicans hyphal morphogenesis target endocytosis. Sci Rep. 2017 07 18; 7(1):5692. View in: PubMed

  43. Holland KM, Rosa SJ, Kristjansdottir K, Wolfgeher D, Franz BJ, Zarrella TM, Kumar S, Sunagar R, Singh A, Bakshi CS, Namjoshi P, Barry EM, Sellati TJ, Kron SJ, Gosselin EJ, Reed DS, Hazlett KRO. Differential Growth of Francisella tularensis, Which Alters Expression of Virulence Factors, Dominant Antigens, and Surface-Carbohydrate Synthases, Governs the Apparent Virulence of Ft SchuS4 to Immunized Animals. Front Microbiol. 2017; 8:1158. View in: PubMed

  44. Skowron KB, Pitroda SP, Namm JP, Balogun O, Beckett MA, Zenner ML, Fayanju O, Huang X, Fernandez C, Zheng W, Qiao G, Chin R, Kron SJ, Khodarev NN, Posner MC, Steinberg GD, Weichselbaum RR. Basal Tumor Cell Isolation and Patient-Derived Xenograft Engraftment Identify High-Risk Clinical Bladder Cancers. Sci Rep. 2016 10 24; 6:35854. View in: PubMed

  45. Flor AC, Kron SJ. Lipid-derived reactive aldehydes link oxidative stress to cell senescence. Cell Death Dis. 2016 09 08; 7(9):e2366. View in: PubMed

  46. Appelbe OK, Zhang Q, Pelizzari CA, Weichselbaum RR, Kron SJ. Image-Guided Radiotherapy Targets Macromolecules through Altering the Tumor Microenvironment. Mol Pharm. 2016 10 03; 13(10):3457-3467. View in: PubMed

  47. Flor AC, Doshi AP, Kron SJ. Modulation of therapy-induced senescence by reactive lipid aldehydes. Cell Death Discov. 2016; 2. View in: PubMed

  48. Labay E, Mauceri HJ, Efimova EV, Flor AC, Sutton HG, Kron SJ, Weichselbaum RR. Repurposing cephalosporin antibiotics as pro-senescent radiosensitizers. Oncotarget. 2016 Jun 07; 7(23):33919-33. View in: PubMed

  49. Duan X, He C, Kron SJ, Lin W. Nanoparticle formulations of cisplatin for cancer therapy. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2016 09; 8(5):776-91. View in: PubMed

  50. Woodford MR, Truman AW, Dunn DM, Jensen SM, Cotran R, Bullard R, Abouelleil M, Beebe K, Wolfgeher D, Wierzbicki S, Post DE, Caza T, Tsutsumi S, Panaretou B, Kron SJ, Trepel JB, Landas S, Prodromou C, Shapiro O, Stetler-Stevenson WG, Bourboulia D, Neckers L, Bratslavsky G, Mollapour M. Mps1 Mediated Phosphorylation of Hsp90 Confers Renal Cell Carcinoma Sensitivity and Selectivity to Hsp90 Inhibitors. Cell Rep. 2016 Feb 02; 14(4):872-884. View in: PubMed

  51. Wolfgeher D, Dunn DM, Woodford MR, Bourboulia D, Bratslavsky G, Mollapour M, Kron SJ, Truman AW. The dynamic interactome of human Aha1 upon Y223 phosphorylation. Data Brief. 2015 Dec; 5:752-5. View in: PubMed

  52. Lee J, Manning AJ, Wolfgeher D, Jelenska J, Cavanaugh KA, Xu H, Fernandez SM, Michelmore RW, Kron SJ, Greenberg JT. Acetylation of an NB-LRR Plant Immune-Effector Complex Suppresses Immunity. Cell Rep. 2015 Nov 24; 13(8):1670-82. View in: PubMed

  53. Efimova EV, Takahashi S, Shamsi NA, Wu D, Labay E, Ulanovskaya OA, Weichselbaum RR, Kozmin SA, Kron SJ. Linking Cancer Metabolism to DNA Repair and Accelerated Senescence. Mol Cancer Res. 2016 Feb; 14(2):173-84. View in: PubMed

  54. Dunn DM, Woodford MR, Truman AW, Jensen SM, Schulman J, Caza T, Remillard TC, Loiselle D, Wolfgeher D, Blagg BS, Franco L, Haystead TA, Daturpalli S, Mayer MP, Trepel JB, Morgan RM, Prodromou C, Kron SJ, Panaretou B, Stetler-Stevenson WG, Landas SK, Neckers L, Bratslavsky G, Bourboulia D, Mollapour M. c-Abl Mediated Tyrosine Phosphorylation of Aha1 Activates Its Co-chaperone Function in Cancer Cells. Cell Rep. 2015 Aug 11; 12(6):1006-18. View in: PubMed

  55. Truman AW, Kristjansdottir K, Wolfgeher D, Ricco N, Mayampurath A, Volchenboum SL, Clotet J, Kron SJ. The quantitative changes in the yeast Hsp70 and Hsp90 interactomes upon DNA damage. Data Brief. 2015 Mar; 2:12-5. View in: PubMed

  56. Labay E, Efimova EV, Quarshie BK, Golden DW, Weichselbaum RR, Kron SJ. Ionizing radiation-induced foci persistence screen to discover enhancers of accelerated senescence. Int J High Throughput Screen. 2011 Mar; 2:1-13. View in: PubMed

  57. Depreux FF, Puckelwartz MJ, Augustynowicz A, Wolfgeher D, Labno CM, Pierre-Louis D, Cicka D, Kron SJ, Holaska J, McNally EM. Disruption of the lamin A and matrin-3 interaction by myopathic LMNA mutations. Hum Mol Genet. 2015 Aug 01; 24(15):4284-95. View in: PubMed

  58. Truman AW, Kristjansdottir K, Wolfgeher D, Ricco N, Mayampurath A, Volchenboum SL, Clotet J, Kron SJ. Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveal chaperone-dependent regulation of ribonucleotide reductase. J Proteomics. 2015 Jan 01; 112:285-300. View in: PubMed

  59. Flor AC, Williams JH, Blaine KM, Duggan RC, Sperling AI, Schwartz DA, Kron SJ. DNA-directed assembly of antibody-fluorophore conjugates for quantitative multiparametric flow cytometry. Chembiochem. 2014 Jan 24; 15(2):267-75. View in: PubMed

  60. Balogun FO, Truman AW, Kron SJ. DNA resection proteins Sgs1 and Exo1 are required for G1 checkpoint activation in budding yeast. DNA Repair (Amst). 2013 Sep; 12(9):751-60. View in: PubMed

  61. Parker LL, Kron SJ. Kinase activation in circulating cells: opportunities for biomarkers for diagnosis and therapeutic monitoring. Expert Opin Med Diagn. 2008 Jan; 2(1):33-46. View in: PubMed

  62. Jiménez J, Truman AW, Menoyo S, Kron SJ, Clotet J. The yin and yang of cyclin control by nutrients. Cell Cycle. 2013 Mar 15; 12(6):865-6. View in: PubMed

  63. Truman AW, Kristjansdottir K, Wolfgeher D, Hasin N, Polier S, Zhang H, Perrett S, Prodromou C, Jones GW, Kron SJ. CDK-dependent Hsp70 Phosphorylation controls G1 cyclin abundance and cell-cycle progression. Cell. 2012 Dec 07; 151(6):1308-18. View in: PubMed

  64. Ghosh G, Yan X, Kron SJ, Palecek SP. Activity assay of epidermal growth factor receptor tyrosine kinase inhibitors in triple-negative breast cancer cells using peptide-conjugated magnetic beads. Assay Drug Dev Technol. 2013 Feb; 11(1):44-51. View in: PubMed

  65. Zhou G, Khan F, Dai Q, Sylvester JE, Kron SJ. Photocleavable peptide-oligonucleotide conjugates for protein kinase assays by MALDI-TOF MS. Mol Biosyst. 2012 Sep; 8(9):2395-404. View in: PubMed

  66. Ghosh G, Lian X, Kron SJ, Palecek SP. Properties of resistant cells generated from lung cancer cell lines treated with EGFR inhibitors. BMC Cancer. 2012 Mar 20; 12:95. View in: PubMed

  67. Meng Y, Efimova EV, Hamzeh KW, Darga TE, Mauceri HJ, Fu YX, Kron SJ, Weichselbaum RR. Radiation-inducible immunotherapy for cancer: senescent tumor cells as a cancer vaccine. Mol Ther. 2012 May; 20(5):1046-55. View in: PubMed

  68. Sylvester JE, Bray TS, Kron SJ. Annotator: postprocessing software for generating function-based signatures from quantitative mass spectrometry. J Proteome Res. 2012 Mar 02; 11(3):1521-36. View in: PubMed

  69. Barreto-Andrade JC, Efimova EV, Mauceri HJ, Beckett MA, Sutton HG, Darga TE, Vokes EE, Posner MC, Kron SJ, Weichselbaum RR. Response of human prostate cancer cells and tumors to combining PARP inhibition with ionizing radiation. Mol Cancer Ther. 2011 Jul; 10(7):1185-93. View in: PubMed

  70. Ulanovskaya OA, Cui J, Kron SJ, Kozmin SA. A pairwise chemical genetic screen identifies new inhibitors of glucose transport. Chem Biol. 2011 Feb 25; 18(2):222-30. View in: PubMed

  71. Zhou G, Yan X, Wu D, Kron SJ. Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MS. Bioconjug Chem. 2010 Oct 20; 21(10):1917-24. View in: PubMed

  72. Rossetto D, Truman AW, Kron SJ, Côté J. Epigenetic modifications in double-strand break DNA damage signaling and repair. Clin Cancer Res. 2010 Sep 15; 16(18):4543-52. View in: PubMed

  73. Zhou G, Sylvester JE, Wu D, Veach DR, Kron SJ. A magnetic bead-based protein kinase assay with dual detection techniques. Anal Biochem. 2011 Jan 01; 408(1):5-11. View in: PubMed

  74. Ghosh G, Yan X, Lee AG, Kron SJ, Palecek SP. Quantifying the sensitivities of EGF receptor (EGFR) tyrosine kinase inhibitors in drug resistant non-small cell lung cancer (NSCLC) cells using hydrogel-based peptide array. Biosens Bioelectron. 2010 Oct 15; 26(2):424-31. View in: PubMed

  75. Efimova EV, Mauceri HJ, Golden DW, Labay E, Bindokas VP, Darga TE, Chakraborty C, Barreto-Andrade JC, Crawley C, Sutton HG, Kron SJ, Weichselbaum RR. Poly(ADP-ribose) polymerase inhibitor induces accelerated senescence in irradiated breast cancer cells and tumors. Cancer Res. 2010 Aug 01; 70(15):6277-82. View in: PubMed

  76. Wu D, Sylvester JE, Parker LL, Zhou G, Kron SJ. Peptide reporters of kinase activity in whole cell lysates. Biopolymers. 2010; 94(4):475-86. View in: PubMed

  77. Sylvester JE, Kron SJ. A bead-based activity screen for small-molecule inhibitors of signal transduction in chronic myelogenous leukemia cells. Mol Cancer Ther. 2010 May; 9(5):1469-81. View in: PubMed

  78. Mand MR, Wu D, Veach DR, Kron SJ. Cell treatment and lysis in 96-well filter-bottom plates for screening Bcr-Abl activity and inhibition in whole-cell extracts. J Biomol Screen. 2010 Apr; 15(4):434-40. View in: PubMed

  79. Nnakwe CC, Altaf M, Côté J, Kron SJ. Dissection of Rad9 BRCT domain function in the mitotic checkpoint response to telomere uncapping. DNA Repair (Amst). 2009 Dec 03; 8(12):1452-61. View in: PubMed

  80. Kim SH, Gadiparthi K, Kron SJ, Kitazono AA. A phosphorylation-independent role for the yeast cyclin-dependent kinase activating kinase Cak1. Gene. 2009 Nov 15; 447(2):97-105. View in: PubMed

  81. Tobe BT, Kitazono AA, Garcia JS, Gerber RA, Bevis BJ, Choy JS, Chasman D, Kron SJ. Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinase. Cell Div. 2009 Jul 01; 4:12. View in: PubMed

  82. Volchenboum SL, Kristjansdottir K, Wolfgeher D, Kron SJ. Rapid validation of Mascot search results via stable isotope labeling, pair picking, and deconvolution of fragmentation patterns. Mol Cell Proteomics. 2009 Aug; 8(8):2011-22. View in: PubMed

  83. Ulanovskaya OA, Janjic J, Suzuki M, Sabharwal SS, Schumacker PT, Kron SJ, Kozmin SA. Synthesis enables identification of the cellular target of leucascandrolide A and neopeltolide. Nat Chem Biol. 2008 Jul; 4(7):418-24. View in: PubMed

  84. Kristjansdottir K, Wolfgeher D, Lucius N, Angulo DS, Kron SJ. Phosphoprotein profiling by PA-GeLC-MS/MS. J Proteome Res. 2008 Jul; 7(7):2812-24. View in: PubMed

  85. Wu D, Mand MR, Veach DR, Parker LL, Clarkson B, Kron SJ. A solid-phase Bcr-Abl kinase assay in 96-well hydrogel plates. Anal Biochem. 2008 Apr 01; 375(1):18-26. View in: PubMed

  86. Bickenbach KA, Veerapong J, Shao MY, Mauceri HJ, Posner MC, Kron SJ, Weichselbaum RR. Resveratrol is an effective inducer of CArG-driven TNF-alpha gene therapy. Cancer Gene Ther. 2008 Mar; 15(3):133-9. View in: PubMed

  87. Parker L, Engel-Hall A, Drew K, Steinhardt G, Helseth DL, Jabon D, McMurry T, Angulo DS, Kron SJ. Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry. J Mass Spectrom. 2008 Apr; 43(4):518-27. View in: PubMed

  88. McSherry TD, Kitazono AA, Javaheri A, Kron SJ, Mueller PR. Non-catalytic function for ATR in the checkpoint response. Cell Cycle. 2007 Aug 15; 6(16):2019-30. View in: PubMed

  89. Parker LL, Brueggemeier SB, Rhee WJ, Wu D, Kent SB, Kron SJ, Palecek SP. Photocleavable peptide hydrogel arrays for MALDI-TOF analysis of kinase activity. Analyst. 2006 Oct; 131(10):1097-104. View in: PubMed

  90. Wysocki R, Javaheri A, Kristjansdottir K, Sha F, Kron SJ. CDK Pho85 targets CDK inhibitor Sic1 to relieve yeast G1 checkpoint arrest after DNA damage. Nat Struct Mol Biol. 2006 Oct; 13(10):908-14. View in: PubMed

  91. Javaheri A, Wysocki R, Jobin-Robitaille O, Altaf M, Côté J, Kron SJ. Yeast G1 DNA damage checkpoint regulation by H2A phosphorylation is independent of chromatin remodeling. Proc Natl Acad Sci U S A. 2006 Sep 12; 103(37):13771-6. View in: PubMed

  92. Parker LL, Kurutz JW, Kent SB, Kron SJ. Control of the yeast cell cycle with a photocleavable alpha-factor analogue. Angew Chem Int Ed Engl. 2006 Sep 25; 45(38):6322-5. View in: PubMed

  93. Kao J, Milano MT, Javaheri A, Garofalo MC, Chmura SJ, Weichselbaum RR, Kron SJ. gamma-H2AX as a therapeutic target for improving the efficacy of radiation therapy. Curr Cancer Drug Targets. 2006 May; 6(3):197-205. View in: PubMed

  94. Kao J, Rosenstein BS, Peters S, Milano MT, Kron SJ. Cellular response to DNA damage. Ann N Y Acad Sci. 2005 Dec; 1066:243-58. View in: PubMed

  95. Wu D, Nair-Gill E, Sher DA, Parker LL, Campbell JM, Siddiqui M, Stock W, Kron SJ. Assaying Bcr-Abl kinase activity and inhibition in whole cell extracts by phosphorylation of substrates immobilized on agarose beads. Anal Biochem. 2005 Dec 01; 347(1):67-76. View in: PubMed

  96. Parker LL, Schilling AB, Kron SJ, Kent SB. Optimizing thiophosphorylation in the presence of competing phosphorylation with MALDI-TOF-MS detection. J Proteome Res. 2005 Sep-Oct; 4(5):1863-6. View in: PubMed

  97. Wysocki R, Javaheri A, Allard S, Sha F, Côté J, Kron SJ. Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9. Mol Cell Biol. 2005 Oct; 25(19):8430-43. View in: PubMed

  98. Brueggemeier SB, Wu D, Kron SJ, Palecek SP. Protein-acrylamide copolymer hydrogels for array-based detection of tyrosine kinase activity from cell lysates. Biomacromolecules. 2005 Sep-Oct; 6(5):2765-75. View in: PubMed

  99. Downs JA, Allard S, Jobin-Robitaille O, Javaheri A, Auger A, Bouchard N, Kron SJ, Jackson SP, Côté J. Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sites. Mol Cell. 2004 Dec 22; 16(6):979-90. View in: PubMed

  100. Wysocki R, Kron SJ. Yeast cell death during DNA damage arrest is independent of caspase or reactive oxygen species. J Cell Biol. 2004 Aug 02; 166(3):311-6. View in: PubMed

  101. Connell PP, Kron SJ, Weichselbaum RR. Relevance and irrelevance of DNA damage response to radiotherapy. DNA Repair (Amst). 2004 Aug-Sep; 3(8-9):1245-51. View in: PubMed

  102. Brueggemeier SB, Kron SJ, Palecek SP. Use of protein-acrylamide copolymer hydrogels for measuring protein concentration and activity. Anal Biochem. 2004 Jun 15; 329(2):180-9. View in: PubMed

  103. Rua D, Holzen T, Glick BS, Kron SJ, Bishop DK. Monitoring changes in the subcellular location of proteins in S. cerevisiae. Methods Mol Biol. 2004; 241:299-311. View in: PubMed

  104. Taneja N, Davis M, Choy JS, Beckett MA, Singh R, Kron SJ, Weichselbaum RR. Histone H2AX phosphorylation as a predictor of radiosensitivity and target for radiotherapy. J Biol Chem. 2004 Jan 16; 279(3):2273-80. View in: PubMed

  105. Sung NS, Gordon JI, Rose GD, Getzoff ED, Kron SJ, Mumford D, Onuchic JN, Scherer NF, Sumners DL, Kopell NJ. Science education. Educating future scientists. Science. 2003 Sep 12; 301(5639):1485. View in: PubMed

  106. Kitazono AA, Garza DA, Kron SJ. Mutations in the yeast cyclin-dependent kinase Cdc28 reveal a role in the spindle assembly checkpoint. Mol Genet Genomics. 2003 Aug; 269(5):672-84. View in: PubMed

  107. Barelle CJ, Bohula EA, Kron SJ, Wessels D, Soll DR, Schäfer A, Brown AJ, Gow NA. Asynchronous cell cycle and asymmetric vacuolar inheritance in true hyphae of Candida albicans. Eukaryot Cell. 2003 Jun; 2(3):398-410. View in: PubMed

  108. LeSage AJ, Kron SJ. Design and implementation of algorithms for focus automation in digital imaging time-lapse microscopy. Cytometry. 2002 Dec 01; 49(4):159-69. View in: PubMed

  109. Choy JS, Kron SJ. NuA4 subunit Yng2 function in intra-S-phase DNA damage response. Mol Cell Biol. 2002 Dec; 22(23):8215-25. View in: PubMed

  110. Kitazono AA, Kron SJ. An essential function of yeast cyclin-dependent kinase Cdc28 maintains chromosome stability. J Biol Chem. 2002 Dec 13; 277(50):48627-34. View in: PubMed

  111. Vander Heiden MG, Choy JS, VanderWeele DJ, Brace JL, Harris MH, Bauer DE, Prange B, Kron SJ, Thompson CB, Rudin CM. Bcl-x(L) complements Saccharomyces cerevisiae genes that facilitate the switch from glycolytic to oxidative metabolism. J Biol Chem. 2002 Nov 22; 277(47):44870-6. View in: PubMed

  112. Palecek SP, Parikh AS, Huh JH, Kron SJ. Depression of Saccharomyces cerevisiae invasive growth on non-glucose carbon sources requires the Snf1 kinase. Mol Microbiol. 2002 Jul; 45(2):453-69. View in: PubMed

  113. Kron SJ. Digital time-lapse microscopy of yeast cell growth. Methods Enzymol. 2002; 351:3-15. View in: PubMed

  114. Gerald JN, Benjamin JM, Kron SJ. Robust G1 checkpoint arrest in budding yeast: dependence on DNA damage signaling and repair. J Cell Sci. 2002 Apr 15; 115(Pt 8):1749-57. View in: PubMed

  115. Palecek SP, Parikh AS, Kron SJ. Sensing, signalling and integrating physical processes during Saccharomyces cerevisiae invasive and filamentous growth. Microbiology (Reading). 2002 Apr; 148(Pt 4):893-907. View in: PubMed

  116. Houseman BT, Huh JH, Kron SJ, Mrksich M. Peptide chips for the quantitative evaluation of protein kinase activity. Nat Biotechnol. 2002 Mar; 20(3):270-4. View in: PubMed

  117. Kitazono AA, Tobe BT, Kalton H, Diamant N, Kron SJ. Marker-fusion PCR for one-step mutagenesis of essential genes in yeast. Yeast. 2002 Jan 30; 19(2):141-9. View in: PubMed

  118. Rua D, Tobe BT, Kron SJ. Cell cycle control of yeast filamentous growth. Curr Opin Microbiol. 2001 Dec; 4(6):720-7. View in: PubMed

  119. Ahn SH, Tobe BT, Fitz Gerald JN, Anderson SL, Acurio A, Kron SJ. Enhanced cell polarity in mutants of the budding yeast cyclin-dependent kinase Cdc28p. Mol Biol Cell. 2001 Nov; 12(11):3589-600. View in: PubMed

  120. Choy JS, Tobe BT, Huh JH, Kron SJ. Yng2p-dependent NuA4 histone H4 acetylation activity is required for mitotic and meiotic progression. J Biol Chem. 2001 Nov 23; 276(47):43653-62. View in: PubMed

  121. Palecek SP, Parikh AS, Kron SJ. Genetic analysis reveals that FLO11 upregulation and cell polarization independently regulate invasive growth in Saccharomyces cerevisiae. Genetics. 2000 Nov; 156(3):1005-23. View in: PubMed

  122. Harris MH, Vander Heiden MG, Kron SJ, Thompson CB. Role of oxidative phosphorylation in Bax toxicity. Mol Cell Biol. 2000 May; 20(10):3590-6. View in: PubMed

  123. Ahn SH, Acurio A, Kron SJ. Regulation of G2/M progression by the STE mitogen-activated protein kinase pathway in budding yeast filamentous growth. Mol Biol Cell. 1999 Oct; 10(10):3301-16. View in: PubMed

  124. Mulet JM, Leube MP, Kron SJ, Rios G, Fink GR, Serrano R. A novel mechanism of ion homeostasis and salt tolerance in yeast: the Hal4 and Hal5 protein kinases modulate the Trk1-Trk2 potassium transporter. Mol Cell Biol. 1999 May; 19(5):3328-37. View in: PubMed

  125. Kron SJ. Filamentous growth in budding yeast. Trends Microbiol. 1997 Nov; 5(11):450-4. View in: PubMed

  126. Zohar AH, Pauls DL, Ratzoni G, Apter A, Dycian A, Binder M, King R, Leckman JF, Kron S, Cohen DJ. Obsessive-compulsive disorder with and without tics in an epidemiological sample of adolescents. Am J Psychiatry. 1997 Feb; 154(2):274-6. View in: PubMed

  127. Cook JG, Bardwell L, Kron SJ, Thorner J. Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev. 1996 Nov 15; 10(22):2831-48. View in: PubMed

  128. Footer M, Egholm M, Kron S, Coull JM, Matsudaira P. Biochemical evidence that a D-loop is part of a four-stranded PNA-DNA bundle. Nickel-mediated cleavage of duplex DNA by a Gly-Gly-His bis-PNA. Biochemistry. 1996 Aug 20; 35(33):10673-9. View in: PubMed

  129. Kron SJ, Gow NA. Budding yeast morphogenesis: signalling, cytoskeleton and cell cycle. Curr Opin Cell Biol. 1995 Dec; 7(6):845-55. View in: PubMed

  130. Kron SJ, Styles CA, Fink GR. Symmetric cell division in pseudohyphae of the yeast Saccharomyces cerevisiae. Mol Biol Cell. 1994 Sep; 5(9):1003-22. View in: PubMed

  131. Ferrando A, Kron SJ, Rios G, Fink GR, Serrano R. Regulation of cation transport in Saccharomyces cerevisiae by the salt tolerance gene HAL3. Mol Cell Biol. 1995 Oct; 15(10):5470-81. View in: PubMed

  132. Kron SJ, Spudich JA. Fluorescent actin filaments move on myosin fixed to a glass surface. Proc Natl Acad Sci U S A. 1986 Sep; 83(17):6272-6. View in: PubMed

  133. Toyoshima YY, Kron SJ, McNally EM, Niebling KR, Toyoshima C, Spudich JA. Myosin subfragment-1 is sufficient to move actin filaments in vitro. Nature. 1987 Aug 6-12; 328(6130):536-9. View in: PubMed

  134. Toyoshima YY, Kron SJ, Spudich JA. The myosin step size: measurement of the unit displacement per ATP hydrolyzed in an in vitro assay. Proc Natl Acad Sci U S A. 1990 Sep; 87(18):7130-4. View in: PubMed

  135. Schwyter DH, Kron SJ, Toyoshima YY, Spudich JA, Reisler E. Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin. J Cell Biol. 1990 Aug; 111(2):465-70. View in: PubMed

  136. Kron SJ, Toyoshima YY, Uyeda TQ, Spudich JA. Assays for actin sliding movement over myosin-coated surfaces. Methods Enzymol. 1991; 196:399-416. View in: PubMed

  137. Kron SJ, Drubin DG, Botstein D, Spudich JA. Yeast actin filaments display ATP-dependent sliding movement over surfaces coated with rabbit muscle myosin. Proc Natl Acad Sci U S A. 1992 May 15; 89(10):4466-70. View in: PubMed