Anindita Basu | Committee on Genetics, Genomics and Systems Biology

Research Summary

We are an interdisciplinary research group that combines expertise from physics, genomics, molecular biology and materials science to study complex biological systems with single cell resolution, at very high throughput, and in context of their native environments. We are jointly affiliated with the department of Medicine at UChicago and Center for Nanoscale Systems at ANL. We develop and apply key technologies that allow single-cell epi-genomic and transcriptomic profiling of as many as 104-105 cells, including mammalian and microbial cells such as bacteria and yeasts, using microfluidics and next-gen sequencing, with applications in basic and translational research. We also develop tools to study inter-cellular interactions in complex biological systems in context of environment, as well as physical and chemical techniques to systematically perturb them. We expect our work will help create better taxonomy of cells in complex tissues, relate epi-genomics to transcription at a single cell level, and decipher inter-cellular interactions in healthy and diseased systems. This will help us understand systems-level cellular organization and function as part of complex biological environments.

Keywords

Single Cell Analysis, Single Nucleus Analysis, Microfluidics, Device Design, Medical, Host Pathogen Interactions, High Throughput RNA Sequencing

Education

Harvard University, Broad Institute, Cambridge, MA, Post-doc Applied Physics, Genomics 10/2016
University of Pennsylvania, Philadelphia, PA, PhD Physics 07/2012
University of Arkansas, Fayetteville, AR, BS Physics; Computer Engineering 05/2004

Biosciences Graduate Program Association

Genetics, Genomics & Systems Biology

Publications

Phan HV, van Gent M, Drayman N, Basu A, Gack MU, Tay S. High-throughput RNA sequencing of paraformaldehyde-fixed single cells. Nat Commun. 2021 09 24; 12(1):5636. View in: PubMed
Hammes M, Moya-Rodriguez A, Bernstein C, Nathan S, Navuluri R, Basu A. Computational modeling of the cephalic arch predicts hemodynamic profiles in patients with brachiocephalic fistula access receiving hemodialysis. PLoS One. 2021; 16(7):e0254016. View in: PubMed
Kaucikas M, Fitzpatrick A, Bryan E, Struve A, Henning R, Kosheleva I, Srajer V, Groenhof G, Van Thor JJ. Room temperature crystal structure of the fast switching M159T mutant of the fluorescent protein dronpa. Proteins. 2015 Mar; 83(3):397-402. View in: PubMed
Van Phan H, van Gent M, Drayman N, Basu A, Gack MU, Tay S. Fixed single-cell RNA sequencing for understanding virus infection and host response. bioRxiv. 2021 Jan 21. View in: PubMed
Guzzetta A, Koska M, Rowton M, Sullivan KR, Jacobs-Li J, Kweon J, Hidalgo H, Eckart H, Hoffmann AD, Back R, Lozano S, Moon AM, Basu A, Bressan M, Pott S, Moskowitz IP. Hedgehog-FGF signaling axis patterns anterior mesoderm during gastrulation. Proc Natl Acad Sci U S A. 2020 07 07; 117(27):15712-15723. View in: PubMed
Selewa A, Dohn R, Eckart H, Lozano S, Xie B, Gauchat E, Elorbany R, Rhodes K, Burnett J, Gilad Y, Pott S, Basu A. Systematic Comparison of High-throughput Single-Cell and Single-Nucleus Transcriptomes during Cardiomyocyte Differentiation. Sci Rep. 2020 01 30; 10(1):1535. View in: PubMed
Hammes M, McGill RL, Basu A, Blicharski T, Delaney K. Hemodynamic effects of hemodialyzer pump speed on arteriovenous fistulas?. Clin Nephrol. 2019 Mar; 91(3):138-146. View in: PubMed
Habib N, Avraham-Davidi I, Basu A, Burks T, Shekhar K, Hofree M, Choudhury SR, Aguet F, Gelfand E, Ardlie K, Weitz DA, Rozenblatt-Rosen O, Zhang F, Regev A. Massively parallel single-nucleus RNA-seq with DroNc-seq. Nat Methods. 2017 Oct; 14(10):955-958. View in: PubMed
Tao Y, Rotem A, Zhang H, Chang CB, Basu A, Kolawole AO, Koehler SA, Ren Y, Lin JS, Pipas JM, Feldman AB, Wobus CE, Weitz DA. Rapid, targeted and culture-free viral infectivity assay in drop-based microfluidics. Lab Chip. 2015 Oct 07; 15(19):3934-40. View in: PubMed
Rotem A, Ram O, Shoresh N, Sperling RA, Schnall-Levin M, Zhang H, Basu A, Bernstein BE, Weitz DA. High-Throughput Single-Cell Labeling (Hi-SCL) for RNA-Seq Using Drop-Based Microfluidics. PLoS One. 2015; 10(5):e0116328. View in: PubMed
Macosko EZ, Basu A, Satija R, Nemesh J, Shekhar K, Goldman M, Tirosh I, Bialas AR, Kamitaki N, Martersteck EM, Trombetta JJ, Weitz DA, Sanes JR, Shalek AK, Regev A, McCarroll SA. Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell. 2015 May 21; 161(5):1202-1214. View in: PubMed
Chen J, Le S, Basu A, Chazin WJ, Yan J. Mechanochemical regulations of RPA's binding to ssDNA. Sci Rep. 2015 Mar 19; 5:9296. View in: PubMed